pbabe puro vector  (Addgene inc)

Journal: Frontiers in Aging

Article Title: Membrane transporter progressive ankylosis protein homologue ( ANKH / Ank ) partially mediates senescence-derived extracellular citrate and is regulated by DNA damage, inflammation, and ageing

doi: 10.3389/fragi.2025.1583288

Figure Lengend Snippet: ANKH transcript is upregulated in PEsen human fibroblasts and downregulated by telomerase. (A) Expression of ANKH mRNA in growing and PEsen NHOF-1 cells using primer set 1. (B) This is similar to (A) but shows the results of primer set 2 in the same experiments. (C) This is similar to (A) but shows the expression of the senescence marker p16INK4A in the same experiments. (D) This is similar to (A) but shows the expression of the senescence marker p21WAF1 in the same experiments. (E) This is similar to (A) but shows SA-βGal expression. (A–E) Yellow plain bars, growing young cells 20.2-21 MPDs; blue horizontal striped bars, PEsen cells 68.2 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; *P > 0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 3. (F) Expression of ANKH mRNA in NHOF-1 PURO, TERT-HA, DNTERT, and TERT cell using primer set 1. N = 3. (G) This is similar to (F) but shows the results of primer set 2 in the same experiments. (H) This is similar to (F) but shows the expression of the senescence marker p16INK4A in the same experiments. (I) This is similar to (F) but shows the expression of the senescence marker p21WAF1 in the same experiments. (J) This is similar to (F) but shows SA-βGal expression. (K,L) Representative images of SA-βGal staining in (E). (K) Young growing NHOF-1 fibroblasts (20.2 MPDs). (L) . NHOF-1 cells (68.2 MPDs). Bar, 100 µm. (F–J) (A–E) Plain yellow bars, LATE PURO; blue horizontal striped bars, TERT-HA; brown stippled bars, DNTERT; and purple left-right diagonally striped bars, TERT all after completing 60 MPDs. *, P < 0.05; **, P < 0.01; ***, P < 0.001; * >0.05 < 0.1; ns, not significant. The results are presented as the averages +/- standard deviation. N = 4, except (J) .

Article Snippet: pBABE retroviral vectors on the puromycin-resistant backbone expressing TERT and TERT-HA , the dominant-negative catalytically dead TERT DNTERT , or the empty vector were obtained from Addgene Europe (Teddington, Middlesex, UK; Cat# 1771, 1772 and 1775, respectively).

Techniques: Expressing, Marker, Standard Deviation, Staining

Journal: eLife

Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models

doi: 10.7554/eLife.98348

Figure Lengend Snippet:

Article Snippet: At day 50, DAN were transduced with a lentiviral vector expressing mRuby2-T2A-GCaMP6f (Addgene plasmid # 197595).

Techniques: Knock-Out, Knock-In, Control, Mutagenesis, Transfection, Construct, Plasmid Preparation, Expressing, Imaging, Recombinant, Sequencing, Multiplex Assay, Software